Salve Meeting 2003 Eyre

Anatomical identification of neuronal circuits

Mark D. Eyre

Attila I. Gulyás
Lászlo Ácsády
Institute of Experimental Medicine
Hungarian Academy of Sciences

Brain anatomy	53.46 KB
Neuronal anatomy	75.70 KB
Modern Tracers 3H Radiolabelled amino-acids Lipophillic dyes e.g. Di I Wheat Germ Agglutin (WGA) Phaseolus lectin (PHAL) Biotinylated Dextran Amine (BDA) Choleratoxin subunit B Toxin tracing using Saporin	39.74 KB
Visualisation of pathways	51.45 KB
Visualisation of axon terminals	35.90 KB
Fluorescent labelling	54.83 KB
Electron Microscopy	60.38 KB
	61.16 KB
Identification of different axon terminals	76.16 KB
Intracellular labelling	62.91 KB
Correlated microscopy	41.59 KB
	49.17 KB
Transneuronal Tracing	44.46 KB
Circuits	57.47 KB
Retina, Cortex	54.89 KB
Hippocampal microcircuits	68.00 KB
	38.25 KB
	42.66 KB

Summary

Modern tracing methods allow specified connections to be investigated
Immunohistochemistry enables afferent (input) and efferent (output) connections to be characterized
Fluorescence microscopy enables more information to be gained about cells and connections, for example neurotransmitter type
Correlated Light- and Electron Microscopy is used to demonstrate synaptic connections and determine their ultrastructural details
Intracellular labelling can trace the precise connections of cells, both from brain area to brain area, and also between different cell types in the same brain structure